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Study of geographical authenticity of Moravian white wines
Horáková, Kristýna ; Křikala, Jakub (referee) ; Diviš, Pavel (advisor)
The aim of this bachelor thesis was to verify geographical autenticity of Moravian white wines based on elemental analysis. This data were completed with the analysis of organic acids, fenolic compounds and atioxidants in wines. There were used two spektrometric methods for the elemental analysis (ICP-MS and ICP-OES). The concentration of organic acids was mesured by Ion chromatography. To determine the concentration of phenolic compounds in wines there was used the high performance liquid chromatography (HPLC-DAD), the content of antioxidants and the total content of phenols was measured on spectrophotometer. In total there were 33 samples of wine from 4 Moravian subregions. For the purpose of determination the samples in groups the dataset was firstly corrected by Grubbs test for remote results. From those data the box plots were created which represents the minimal, maximal and mean concentrations of each measured component. Furthermore the analysis of variance was applied, which picked up 12 statisticly valuable parameters from the whole 47. By appliyng the multidimensional statistical methods (Pearson correlation analysis (PCA) and discriminant analysis (DA)) were compiled mathematical models for the division of Moravian wines according to geographical origin. The success rate of the discrimination test was 96,97%. The cross-validation test showed an 81,82% success rate for classifying unknown samples.
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Study of geographical authenticity of moravian red wine
Bidmonová, Karolína ; Punčochářová, Lenka (referee) ; Pořízka, Jaromír (advisor)
This bachelor thesis discusses the possibility of using analytical methods to determine the elemental profile of wine and to detect phenolic substances in wine. The theoretical part describes the Czech and Moravian subregions, chemical composition of wine, authenticity and methods of assessing the authenticity of wine. The next part contains a brief description of the analytical methods that were used in the experimental part. The experimental part deals with the determination of the elemental profile of wine by mass and optical emission spectrometry, the determination of phenolic substances by high performance liquid chromatography, the determination of antioxidant activity and the total content of phenolic substances. For the purpose of classifying wine samples into groups, multidimensional statistical methods (cluster analysis, principal component analysis, analysis of variance and discriminant analysis) were used to separate the groups according to geographical origin.
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Refinery and characterization of grape oil
Prchal, Miroslav ; Vítová, Eva (referee) ; Kovalčík, Adriána (advisor)
This bachelor thesis deals with the topic concerning the extraction of wine oil from grape seeds, the refining of the extracted oil and the characterization of grape oil. Grape oil was extracted from the grape seeds obtained from the harvest in September 2019 by the winery Vavricek (Brezi u Mikulova, Czech Republic). The received grape pomace was the mixture of Green Veltliner and Blanc Saugvinon. The oil was extracted from the separated seeds by using liquid extraction in n-Hexane. The extraction yields of grape oil were compared with the literature, involving the different extraction methods as well as different oil plants. For the refining of the grape oil have been applied more steps aiming for the removal of the lipophilic pigments. Different adsorbents of pigments have been applied. The purification efficiency has been proved by the investigation with the thin layer and the high-performance chromatography. Moreover, the gas chromatography was used to characterize the composition of the oil. Finally, the composition profile of the oil obtained by the liquid extraction before and after purification has been compared with the commercial grape oil, which has been received by cold pressing. The results of the work show that the winery waste produced in South Moravia is a valuable raw material, which can be used for the production and different products, e.g., grape oil and pigments (chlorophyll).
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Possibilities of using of Vitis vinifera as a source of raw materials for cosmetic production
Hanáková, Pavlína ; Vítová, Eva (referee) ; Zemanová, Jana (advisor)
This bachelor thesis deals with preparation of extraction from various parts of grapevine and their consequent use in the manufacture of cosmetics. The theoretical part is focused on general characteristics of grapevine, its taxonomic classification, occurrence and cultivation. Subsequently, the chemical composition of grapevine parts and their uses in cosmetic, food and pharmaceutical industries is described. The final part is devoted to particular types of extractions and methods of determinativ of total phenolic substances. The experimental part deals with the optimization of the prepared extract in order to obtain the extract with the highest amount of total phenolic substances with respect to production costs. Factors such as selection of the appropriate solvent and extraction time are examined. 50% ethanol at the room temperature was found to be an optimal solvent. Finally, the physical and chemical properties, such as density, pH and refractive index were determined for the purpose of producing a material safety data sheet. The extract was processed into cosmetic products.
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Application of analytical techniques and chemometry for authentification of wine
Pořízka, Jaromír ; Karovičová, Jolana (referee) ; Jarošová, Alžběta (referee) ; Diviš, Pavel (advisor)
This PhD. thesis is focused on application of different analytical techniques for determination of elemental composition of Moravian wine combined with analysis of selected polyphenol compounds. Two spectrometric techniques were used for elemental analysis of wine – Mass spectrometry and Optical emission spectrometry with inductively coupled plasma (ICP-MS and ICP-OES). Analysis of organic constituents of wine was evaluated by High performance liquid chromatograph with diode array detector (HPLC-DAD). Data from analysis of wine were used for construction of mathematical models for determining geographical origin of wine and for determining type of varietal wine. Data handling was performed by usage of multivariate statistical methods (cluster analysis, principal component analysis and canonical discriminant analysis). For the discrimination of wine by the geographical origin, two separate models for white and red wine were constructed. Effectiveness of discrimination of Moravian wine (with knowledge about origin) into 4 wine regions was 100 % successful for white wine and 96,43 % for red wine samples. Those models were used for cross validation test of unknown samples with result 95 % successfully classified samples of white wine and 82,15% of red wine. Besides that, mathematical model for discrimination and classification of varietal wine was made. Müller Thurgau, Riesling and Gruner Veltliner were discriminated by the usage of 8 elemental parameters. Discrimination success rate of known samples was evaluated by canonical discriminant analysis with 95,83% of correctly classified known samples and 70,83% of correctly classified unknown varietal wine (cross validation test). In addition to these part of research, impact of different grape production systems on elemental composition of wine and Vitis Vinifera was studied. The core of the study was to find the connections between the type of agriculture and chemical properties of wine and Vitis Vinifera. Two agriculture systems were assessed – Organic agriculture and Integrated pest management. . Results of this study showed that quantity and manner of pesticide application is very important factor affecting the final elemental composition of the grapevine and wine, especially the content of copper, zinc and manganese. That knowledge can be used in the future for creation of classification model of wine according to grape production system. For comparison of radical scavenging activity, electron paramagnetic resonance spectroscopy (EPR) with DPPH method was used. In the case of this study, the influence of vine growing under different farming systems on the total antioxidant activity of wine was found to be insignificant.
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Zhodnocení významu škod zvěří na vinicích v okolí obce Limbach
Hlavatovič, Ondrej
This bachelor thesis deals with the assessment of the importance of wildlife damage in vineyards. The vineyards on which the field investigations were carried out are located in the vicinity of the village of Limbach, located in the Pezinok district. The actual damage assessment was carried out using the walking method on three areas of interest using bite plots located at the beginning, middle and end of the vineyard. Subsequently, the economic impact of the damage on the individual areas of interest was quantified. The work also included monitoring of the intensity of vineyard use by wildlife, which was carried out using photo traps placed at the points of arrival of wildlife in the vineyard.
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Mechanizace sklizně vinné révy
Zborovský, Miroslav
The bachelor thesis deals with the mechanization of grape harvest in viticulture. It describes the development of mechanical grape harvest and focuses on specific types of machines currently available on the market as well as their historical background. The agrotechnical requirements necessary for establishing a vineyard with a vision for future mechanized operations are also evaluated. Using data from professional literature and technical specifications, relevant parameters that grape harvesting machines must meet are determined, followed by an assessment of two machines from the brands PELLENC and NEW HOLLAND.
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